Lung fluorescence imaging to evaluate tumor metastasis induced by AQP5 expression in murine model.

Abstract

Our recent study, by up-regulation of AQP5 expression, showed enhanced proliferation and migration potential in lung cancer. However, so far none of the in vivo study of gene silencing of AQP5 has been tested. In this study, we tested roles of AQP5 on lung cancer metastasis potential by gene silencing of AQP5 in two lung cancer cell lines and tried to monitor lung metastases with EGFP marker. Lungs were imaged at different time points and allowed an accurate evaluation of tumor burden over time. Our results showed significantly decreased metastasis potential in AQP5 gene-silencing cells. Lung imaging confirmed the frequency of metastasis in mice. These data provide more evidence that AQP5 plays important roles in the metastasis potential of lung cancer. Lung fluorescence imaging provides rapid monitoring for tumor growth and metastasis, and it also offers quantitative and sensitive analysis of tumor growth and metastasis, compared to the traditional histology technique.