Peer-reviewed veterinary case report
How dog immune cells react to ovalbumin allergy testing
By Dévaud, N et al.·Published in Veterinary journal (London, England : 1997)·2009·Department of Clinical Veterinary Medicine·View original on PubMed →
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Original publication title: Lymphocyte blastogenic response to ovalbumin in a model for canine allergy.
- Species:
- dog
Plain-English summary
Five dogs with allergies to ovalbumin (a protein found in egg whites) were tested to see how their immune cells reacted to this allergen. After being exposed to ovalbumin eye drops, the dogs showed increased redness in their eyes, indicating an allergic response. The tests revealed that the allergic dogs had specific immune cells that reacted to ovalbumin, which were not present in healthy dogs. This study suggests that a special test can help identify immune responses in dogs with allergies, even when they are not currently exposed to the allergen.
People also search for: dog eye allergy treatment · why is my dog’s eye red · ovalbumin allergy in dogs
Abstract
Lymphocyte stimulation tests (LST) were performed in five dogs sensitised with ovalbumin (OVA) and seven healthy dogs. In addition, all five OVA-sensitised and two control dogs were tested after two in vivo provocations with OVA-containing eye drops. The isolated cells were suspended in culture media containing OVA and were cultured for up to 12 days. Proliferation was measured as reduction in 5,6-carboxylfluorescein diacetate succinimidyl ester (CFSE) intensity by flow cytometry on days 0, 3, 6, 9 and 12. A cell proliferation index (CPI) for each day and the area under the curve (AUC) of the CPI was calculated for each dog. All OVA-sensitised dogs demonstrated increased erythema after conjunctival OVA application. The presence of OVA-specific lymphocytes was demonstrated in 2/5 OVA-sensitised dogs before and 4/5 after in vivo provocation. Using the AUC, the difference between OVA-sensitised and control dogs was significant in all three LST before in vivo provocation (P<0.05) and borderline significant (P=0.053) in 2/3 LST after provocation. The most significant difference in CPI was observed after 9 days of culture (P=0.001). This pilot study indicates that the LST allows detection of rare antigen specific memory T-cells in dogs previously sensitised to, but not concurrently undergoing challenge by a specific antigen.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/18397835/