Magnetically-Induced Suppression of Oxidative Stress Prevents Venous Thrombosis.

Abstract

Venous thrombosis remains a major clinical challenge, with current therapeutic options limited by bleeding risks and procedural invasiveness. Here, it is demonstrated that static magnetic fields (SMFs) provide a non-invasive and effective strategy for thrombosis prevention by mitigating oxidative damage to the vascular endothelium. In a murine model induced by FeCl, exposure to SMFs significantly reduces thrombus formation, improves survival, and restores venous blood flow without affecting systemic coagulation or fibrinolytic pathways. Mechanistically, SMFs suppressed ROS accumulation and endothelial apoptosis in HO-challenged vascular cells by elevating intracellular Calevels, which promotes ATP synthesis and suppress NOX4-mediated oxidative stress. Furthermore, the protective effects of SMF against oxidative stress are largely diminished in the presence of either the calcium channel blocker or the specific NOX4 enzyme inhibitor. These findings reveal a Ca-ATP-NOX4 signaling axis as a key mediator of the vascular-protective effects of SMFs, establishing a mechanistic rationale for their antithrombotic action. The consistent efficacy observed across varying SMF intensities underscores their translational potential as a next-generation thromboprophylactic modality.