Peer-reviewed veterinary case report
Malassezia otitis externa in the dog: the effect of heat-fixing otic exudate for cytological analysis.
- Journal:
- Journal of veterinary medicine. A, Physiology, pathology, clinical medicine
- Year:
- 2007
- Authors:
- Griffin, J S et al.
- Affiliation:
- Department of Clinical Sciences · United States
- Species:
- dog
Plain-English summary
This study looked at 32 dogs with a condition called Malassezia otitis externa, which is an ear infection caused by a type of yeast. The researchers wanted to see if heating the ear discharge before examining it under a microscope would change the results. They collected samples from each dog and prepared two slides: one was heated and the other was not. After examining both slides, they found that heating the samples did not consistently change the number of yeast cells seen in the ear discharge. Overall, the treatment did not show a significant difference in the results.
Abstract
This study was conducted on 32 dogs with Malassezia otitis externa to determine the effect of heat-fixing otic exudate on cytological analysis. Malassezia infection was confirmed by cytological examination of otic exudate. Otic discharge collected with cotton swabs was then rolled onto glass slides. One slide per dog was heat-fixed prior to staining; the other slide was not heat-fixed. The number of yeast in 10 oil-immersion fields (1000 x magnification) was counted for both slides from each dog. Heat-fixing did not systematically cause either increased or decreased numbers of Malassezia on cytology of otic exudate.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/17877584/