Peer-reviewed veterinary case report
Serum peptide patterns may help diagnose canine hepatozoonosis
By Lattisarapunt, Kajornsak et al.·Published in Scientific reports·2025·Pathobiology program Faculty of Veterinary Science·View original on PubMed →
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Original publication title: Mass spectrometry-based serum peptidomic profiling reveals potential biomarker for canine hepatozoonosis.
- Species:
- dog
Plain-English summary
A group of dogs infected with Hepatozoon canis, a parasite that can cause serious illness, was studied to find new ways to diagnose the disease. Researchers compared blood samples from infected dogs to healthy ones and discovered five potential peptide markers that could help identify the infection. This study is the first to use a specific blood analysis technique to look for these markers, which could lead to better diagnosis and treatment options for dogs suffering from this condition.
People also search for: dog hepatozoonosis symptoms · canine vector-borne disease diagnosis · blood test for dog parasites
Abstract
Canine hepatozoonosis, caused by Hepatozoon canis, is a significant canine vector-borne disease (CVBD) with a complex life cycle and diverse clinical manifestations, ranging from asymptomatic to severe illness. While several diagnostic methods are available, no studies have yet applied serum peptidomic analysis to this disease. This study aimed to identify peptide mass fingerprints (PMFs), serum peptidome clusters, and potential biomarker peptides in infected dogs. Blood and serum samples were collected from two groups: H. canis-infected dogs (n = 15) and healthy controls (n = 20). Serum peptidome profiling was conducted using MALDI-TOF MS and LC-MS/MS, with data analyzed via MetaboAnalyst 6.0. Distinct PMFs from MALDI-TOF MS effectively distinguished between infected and control groups, revealing five potential peptide markers (m/z 2892.06, 2837.65, 2874.76, 1495.25, and 1423.79). LC-MS/MS identified 98 upregulated peptides in the infected group. Protein interaction analysis highlighted TNS1, ZEB2, and mTOR, suggesting links to potential therapeutic targets. This is the first study to apply a peptidomic approach to canine hepatozoonosis, demonstrating its value in identifying novel biomarker panels and offering a promising diagnostic tool for improved disease detection.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/41023322/