Peer-reviewed veterinary case report
mRNA levels in duodenal tissue of dogs with chronic diarrhea compared
By Peters, Iain R et al.·Published in American journal of veterinary research·2005·School of Clinical Veterinary Science, United Kingdom·View original on PubMed →
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Original publication title: Measurement of messenger RNA encoding the alpha-chain, polymeric immunoglobulin receptor, and J-chain in duodenal mucosa from dogs with and without chronic diarrhea by use of quantitative real-time reverse transcription-polymerase chain reaction assays.
- Species:
- dog
Plain-English summary
A group of dogs, including 12 German Shepherds, were evaluated for chronic diarrhea, and biopsies were taken from their intestines to study gene expression related to immune function. The researchers found no significant differences in the levels of certain immune-related genes between the dogs with diarrhea and those without. This suggests that the chronic diarrhea seen in German Shepherds is not due to a failure in the genes responsible for producing important immune molecules. Understanding these findings can help veterinarians better address chronic diarrhea in dogs.
People also search for: dog chronic diarrhea treatment · German Shepherd diarrhea causes · intestinal biopsy results in dogs
Abstract
OBJECTIVE: To examine the difference in expression of messenger RNA (mRNA) transcripts for polymeric immunoglobulin receptor (plgR), alpha-chain, and J-chain determined by use of quantitative real-time reverse transcription-polymerase chain reaction (QRT-PCR) assays in duodenal biopsy specimens obtained from dogs with and without chronic diarrhea. SAMPLE POPULATION: Biopsy specimens of the proximal portion of the duodenum were obtained endoscopically from 39 dogs evaluated because of chronic diarrhea (12 German Shepherd Dogs and 27 non-German Shepherd Dog breeds); specimens were also obtained from a control group of 7 dogs evaluated because of other gastrointestinal tract diseases and 2 dogs that were euthanatized as a result of nongastrointestinal tract disease. PROCEDURE: Dogs were anesthetized, and multiple mucosal biopsy specimens were obtained endoscopically at the level of the caudal duodenal flexure by use of biopsy forceps; in 2 control dogs, samples were obtained from the descending duodenum within 5 minutes of euthanasia. One-step QRT-PCR was used to quantify the level of expression of transcripts for the housekeeper gene glyceraldehyde-3-phosphate dehydrogenase, plgR, alpha-chain, and J-chain in duodenal mucosal tissue. RESULTS: There was no significant difference in the level of expression of any transcript among non-German Shepherd Dog breeds without diarrhea (control group), non-German Shepherd Dog breeds with chronic diarrhea, and German Shepherd Dogs with chronic diarrhea. Conclusions and Clinical Relevance-Results indicated that the susceptibility of German Shepherd Dogs to chronic diarrhea is not a result of simple failure of transcription of the key genes that encode molecules involved in mucosal IgA secretion.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/15691029/