Peer-reviewed veterinary case report
Methicillin resistance in staph from dogs with skin pyoderma
By Kania, Stephen A et al.·Published in American journal of veterinary research·2004·Department of Comparative Medicine, United States·View original on PubMed →
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Original publication title: Methicillin resistance of staphylococci isolated from the skin of dogs with pyoderma.
- Species:
- dog
Plain-English summary
A study looked at skin infections (pyoderma) in 90 dogs and found that most staph bacteria causing these infections were still treatable with common antibiotics. However, about half of the bacteria had a gene that could make them resistant to methicillin, a stronger antibiotic. While only a few of the Staphylococcus intermedius bacteria were resistant, many of the Staphylococcus schleiferi bacteria showed significant resistance. This information can help veterinarians understand and manage antibiotic resistance in dogs with skin infections better.
People also search for: dog skin infection treatment · pyoderma in dogs · methicillin-resistant staph in dogs
Abstract
OBJECTIVE: To determine the methicillin-resistant profile of staphylococcal isolates from the skin of dogs with pyoderma. ANIMALS: 90 dogs with pyoderma. PROCEDURE: Staphylococci isolated from dogs with pyoderma were tested for susceptibility to methicillin by use of a standard disk diffusion test with oxacillin disks. The DNA extracted from the isolates was tested for the mecA gene that encodes the penicillin-binding protein 2a (PBP2a) by use of a polymerase chain reaction (PCR) assay. The expression of PBP2a was determined with a commercial latex agglutination assay. Species of staphylococcal isolates were identified by use of morphologic, biochemical, and enzymatic tests. RESULTS: Most of the isolated staphylococci were methicillin-susceptible, coagulase-positive Staphylococcus intermedius isolates. Whereas only 2 of 57 S. intermedius isolates were resistant to methicillin, approximately half of the isolates had the mecA gene and produced PBP2a. Staphylococcus schleiferi was the second most common isolate. Widespread resistance to methicillin was found among S. schleiferi isolates. More coagulase-negative S. schleiferi isolates were identified with mecA gene-mediated resistance to methicillin, compared with coagulase-positive S. schleiferi isolates. CONCLUSIONS AND CLINICAL RELEVANCE: The latex agglutination assay for the detection of PBP2a expression coupled with the PCR assay for the mecA gene may provide new information about emerging antimicrobial resistance among staphylococcal isolates.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/15478775/