Peer-reviewed veterinary case report
Methods for enhanced culture recovery of Francisella tularensis.
- Journal:
- Applied and environmental microbiology
- Year:
- 2004
- Authors:
- Petersen, Jeannine M et al.
- Affiliation:
- Diagnostic and Reference Section · United States
Abstract
Francisella tularensis is found in a wide variety of hosts and extrahost environments, making culture recovery a diagnostic challenge. Here we demonstrate improved recovery times and good sensitivity (90%) when cultures were inoculated on the site of an investigation using fresh tissues. For contaminated specimens, antibiotic supplementation of enriched cysteine heart agar blood culture medium improved recovery of F. tularensis by 81.1%. For transport of tissues, immediate freezing yielded culture recovery rates as high as 94%.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/15184180/