Methods for experimentally increasing circulating acyl-CoA-binding protein (ACBP) levels in mice under chronic restraint stress.

Abstract

Chronic restraint stress (CRS) is a widely recognized model to study stress-induced anorexia and metabolic dysregulation in mice. Acyl-coenzyme A-binding protein (ACBP) has emerged as a critical player in metabolic regulation, with potential implications for stress-related disorders. This study presents two complementary methodologies to artificially elevate circulating Acyl-CoA-binding protein (ACBP) levels in mice under CRS. The first approach involves the continuous delivery of recombinant ACBP via subcutaneously implanted osmotic pumps. The second approach utilizes the retention using selective hooks (RUSH) system, a chemical-genetic platform enabling controlled secretion of ACBP through a biotin-activated mechanism. These methodologies aim to counteract the metabolic and behavioral impacts of CRS, offering a framework for investigating ACBP's therapeutic potential in mitigating anorexia and restoring metabolic homeostasis. The integration of these delivery systems provides a robust tool for advancing research on stress-related disorders.