Peer-reviewed veterinary case report
Methods for growing and titrating African swine fever virus: field and laboratory samples.
- Journal:
- Current protocols in cell biology
- Year:
- 2011
- Authors:
- Carrascosa, Angel L et al.
- Affiliation:
- Centro de Biologia Molecular "Severo Ochoa" (CSIC-UAM) · Spain
Abstract
Growing African swine fever virus (ASFV) isolates obtained mainly from the field, but also engineered in the laboratory, is a critical step for diagnosis, titration, or virus infection studies. This unit describes a set of methods and protocols to produce and titrate any ASFV strain in cell cultures. The procedures include (1) basic techniques to prepare virus-sensitive target cells; (2) strategies for growth, concentration, and purification of virus stocks; and (3) the semi-quantitative (end dilution) and quantitative (plaque) assays for the determination of viral titers, and the use of different ASFV-sensitive cells as targets for virus production and titration.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22161547/