Peer-reviewed veterinary case report
Gene changes in uterus of dogs with pyometra infection
By Bukowska, D et al.·Published in Journal of biological regulators and homeostatic agents·2014·Institute of Veterinary Sciences·View original on PubMed →
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Original publication title: Microarray analysis of inflammatory response-related gene expression in the uteri of dogs with pyometra.
- Species:
- dog
Plain-English summary
A group of 23 female dogs with pyometra, a serious infection of the uterus, showed significantly increased levels of certain inflammatory markers compared to healthy dogs. Symptoms of pyometra can include lethargy, increased thirst, and a foul-smelling discharge. The study identified specific genes that were much more active in the infected dogs, suggesting these could be used as markers for diagnosing the condition. While the research focused on gene expression, it highlights the importance of recognizing pyometra early, as it can be life-threatening. Treatment typically involves surgery to remove the infected uterus.
People also search for: dog pyometra symptoms · how to treat pyometra in dogs · signs of infection in female dogs
Abstract
Pyometra, which is accompanied by bacterial contamination of the uterus, is defined as a complex disease associated with the activation of several systems, including the immune system. The objective of the study was to evaluate the gene expression profile in dogs with pyometra compared with those that were clinically normal. The study included uteri from 43 mongrel bitches (23 with pyometra, 20 clinically healthy). RNA used for the microarray study was pooled to four separated vials for control and pyometra. A total of 17,138 different transcripts were analyzed on the uteri of female dogs with pyometra and of healthy controls. From 264 inflammatory response-related transcripts, we found 23 transcripts that revealed a 10- to 77-fold increased expression. Thereby, the expression of interleukin 8 (IL8), interleukin-1-beta (IL1B), interleukin 18 receptor (IL18RAP), interleukin 1-alpha (IL1A), interleukin receptor antagonist (IL1RN) and interleukin 6 (IL6) increased 77-, 20-, 17-, 13-, 13- and 11-fold, respectively. Furthermore, the expression of the calcium binding proteins S100A8 was 44-fold higher, and that of S100A12 and S100A9 37-fold, respectively, in the uteri of canines with pyometra compared with that of the controls. Moreover, the expression of the transcripts of toll-like receptors (TLR8 and TLR2), integrin beta 2 (ITGB2), chemokine ligand 3 (CCL3), semaphorin 7A (SEMA7A), CD14 and prostaglandin-endoperoxide synthase 2 (PTGS2) was increased between 10- and 18-fold. Furthermore, after using RT-qPCR we found an increased expression of AOAH, IL1A, IL8, CCL3, IL1RN and SERPINE 1 mRNAs which can be served also as markers of the occurrence of pyometra in domestic bitches. In summary, it is concluded that up-regulation of interleukins may be used as a marker of the inflammatory response in dogs with pyometra. Moreover, all of the 23 up-regulated transcripts may be novel molecular markers of the pathogenesis of canine pyometra. Several proteins--–products of these genes--may be recognized as potential biomarkers of this disease or as therapeutic targets in other mammalian species, including humans.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/25620174/