Peer-reviewed veterinary case report
Microfluidic chip-based detection and intraspecies strain discrimination of Salmonella serovars derived from whole blood of septic mice.
- Journal:
- Applied and environmental microbiology
- Year:
- 2013
- Authors:
- Patterson, Adriana S et al.
- Affiliation:
- University of California · United States
- Species:
- rodent
Plain-English summary
Salmonella is a type of bacteria that can cause serious illnesses in both people and animals, leading to conditions like diarrhea and blood infections. Researchers have developed a small, easy-to-use device called a microfluidic chip that can quickly identify different types of Salmonella from blood samples taken from sick mice. This chip works by amplifying the DNA of the bacteria and then detecting it, allowing for results in under two hours. The device was able to accurately distinguish between two specific strains of Salmonella that can cause severe illness in humans. Overall, this new chip shows great potential for quickly diagnosing Salmonella infections in both medical and veterinary settings.
Abstract
Salmonella is a zoonotic pathogen that poses a considerable public health and economic burden in the United States and worldwide. Resultant human diseases range from enterocolitis to bacteremia to sepsis and are acutely dependent on the particular serovar of Salmonella enterica subsp. enterica, which comprises over 99% of human-pathogenic S. enterica isolates. Point-of-care methods for detection and strain discrimination of Salmonella serovars would thus have considerable benefit to medical, veterinary, and field applications that safeguard public health and reduce industry-associated losses. Here we describe a single, disposable microfluidic chip that supports isothermal amplification and sequence-specific detection and discrimination of Salmonella serovars derived from whole blood of septic mice. The integrated microfluidic electrochemical DNA (IMED) chip consists of an amplification chamber that supports loop-mediated isothermal amplification (LAMP), a rapid, single-temperature amplification method as an alternative to PCR that offers advantages in terms of sensitivity, reaction speed, and amplicon yield. The amplification chamber is connected via a microchannel to a detection chamber containing a reagentless, multiplexed (here biplex) sensing array for sequence-specific electrochemical DNA (E-DNA) detection of the LAMP products. Validation of the IMED device was assessed by the detection and discrimination of S. enterica subsp. enterica serovars Typhimurium and Choleraesuis, the causative agents of enterocolitis and sepsis in humans, respectively. IMED chips conferred rapid (under 2 h) detection and discrimination of these strains at clinically relevant levels (<1,000 CFU/ml) from whole, unprocessed blood collected from septic animals. The IMED-based chip assay shows considerable promise as a rapid, inexpensive, and portable point-of-care diagnostic platform for the detection and strain-specific discrimination of microbial pathogens.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23354710/