Peer-reviewed veterinary case report
Microvesicles isolated from 5-azacytidine-and-resveratrol-treated mesenchymal stem cells for the treatment of suspensory ligament injury in horse-a case report.
- Journal:
- Stem cell research & therapy
- Year:
- 2019
- Authors:
- Kornicka-Garbowska, Katarzyna et al.
- Affiliation:
- Department of Experimental Biology
- Species:
- horse
Plain-English summary
In this case, a 6-year-old Dutch Warmblood gelding, which is a type of horse used for jumping, was diagnosed with a suspensory ligament injury, a common cause of lameness in athletic horses. To help heal the injury, the horse was treated with microvesicles, which are tiny particles released from stem cells that had been treated with a combination of two substances called 5-azacytidine and resveratrol. The treatment showed promising results, as it helped fill in the injury and improved blood flow and tissue flexibility in the affected area. This suggests that using microvesicles could be a good alternative to traditional stem cell therapies for treating suspensory ligament injuries in horses.
Abstract
BACKGROUND: In athlete horses, suspensory ligament (SL) injuries are the most common cause of lameness. Healing of SL injury is still problematic, and even proper rehabilitation and pharmacological therapy do not guarantee returning to the initial performance level. In our previous studies, we have shown that a combination of 5-azacytidine (AZA) and resveratrol (RES) exerts beneficial, rejuvenating effects on metabolic syndrome derived adipose-derived stem cells (ASCs). Thus, in the presented research, we investigate whether not only rejuvenated ASC but also microvesicles (MVs) secreted by them possess enhanced regenerative properties in SL injury. METHODS: In the presented study, a 6-year-old Dutch Warmblood gelding, working in jumping, was diagnosed with SL injury using ultrasonography, Doppler, real-time elastography and thermography. As a therapeutic strategy, the affected animal was treated with extracellular microvesicles derived from ASC treated with the combination of 5-azacytydine (AZA) and resveratrol (RES) (MVs)RESULTS: First, anti-apoptotic effects of MVswere tested in co-culture with metabolic syndrome derived ASC. The proliferation of cells and expression of pro-apoptotic genes were investigated. Then, MVswere injected directly into the injured SL of the Dutch Warmblood gelding. In vitro assays revealed that MVsenhance the proliferation of ASC and exert an anti-apoptotic effect. In the affected horse, the application of MVsresulted in increased lesion filling and improvement of angiogenesis and elasticity in injured tissue. CONCLUSIONS: As MVsmimic several of the biological actions exerted by ASC, they have become an alternative for stem cell-based therapies and can be effectively applied for the treatment of SL injury in horses.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/31852535/