Peer-reviewed veterinary case report
Urine microRNA markers for canine bladder cancer diagnosis
By Karttunen, Jenni et al.·Published in Scientific reports·2024·Department of Veterinary Medicine, United Kingdom·View original on PubMed →
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Original publication title: miR-182, miR-221 and miR-222 are potential urinary extracellular vesicle biomarkers for canine urothelial carcinoma.
- Species:
- dog
Plain-English summary
A study found that certain microRNAs (miR-182, miR-221, and miR-222) in the urine of dogs could help diagnose bladder cancer (urothelial carcinoma). Researchers analyzed urine samples from dogs with this cancer and discovered that these specific miRNAs were present in higher amounts compared to healthy dogs and those with urinary tract infections. This suggests that testing for these miRNAs could be a new way to identify bladder cancer in dogs, potentially making diagnosis easier and more accurate.
People also search for: dog bladder cancer symptoms · canine urothelial carcinoma diagnosis · dog urine test for cancer
Abstract
Current diagnostic methods for canine urothelial carcinoma (UC) are technically challenging or can lack specificity, hence there is a need for novel biomarkers of UC. To this end, we analysed the microRNA (miRNA) cargo of extracellular vesicles (EVs) from urine samples of dogs with UC to identify candidate miRNA biomarkers. Urine was fractionated using ultrafiltration combined with size-exclusion chromatography and small RNA sequencing analysis was performed on both the EV enriched and (EV free) protein fractions. A greater number of candidate miRNA biomarkers were detected in the EV fraction than the protein fraction, and further validation using droplet digital PCR (ddPCR) was performed on the EV enriched fraction of a second cohort of dogs with UC which indicated that miR-182, miR-221 and miR-222 were significantly overrepresented in dogs with UC when compared with healthy dogs and dogs with urinary tract infections. Pathway analysis confirmed that these three miRNAs are involved in cancer. In addition, their potential downstream gene targets were predicted and PIK3R1, a well-known oncogene is likely to be a shared target between miRNA-182 and miRNA-221/222. In summary, this study highlights the potential of urinary EV-associated miRNAs as a source of biomarkers for the diagnosis of canine UC.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/39095540/