miR-379-5p retards the proliferation and differentiation of goat skeletal muscle satellite cells by targeting.

Abstract

MicroRNAs (miRNAs) are emerging as crucial regulators of skeletal muscle development and regeneration; however, the biological functions of many miRNAs remain to be elucidated. In this study, we focused on the function of miR-379-5p, a miRNA we previously identified as highly expressed in the longissimus dorsi muscle of goats. Overexpression of miR-379-5p inhibited the proliferation and differentiation of goat skeletal muscle satellite cells (MuSCs), as evidenced by decreased expression of proliferation and differentiation markers, reduced EdUcells, and lower myotube formation. Through bioinformatics prediction and experimental validation, we identifiedas a direct downstream target of miR-379-5p. Functional assays revealed thatpromoted the proliferation and differentiation of MuSCs, whereas miR-379-5p suppressed these processes by decreasingexpression. Furthermore, miR-379-5p inhibited mitochondrial activity during the proliferation phase but promoted it during myogenic differentiation. Additionally, ectopic expression ofdecreased mitochondrial membrane potential and enhanced reactive oxygen species (ROS) production, suggesting thatimpairs mitochondrial function. Overall, our findings highlight the role of miR-379-5p andin regulating goat MuSCs activity and mitochondrial function, providing new insights into the role of miRNAs in skeletal muscle development.