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Peer-reviewed veterinary case report

Modeling Distal Middle Cerebral Artery Occlusion in Neonatal Rodents with Magnetic Nanoparticles or Magnetized Red Blood Cells.

Journal:
Methods in molecular biology (Clifton, N.J.)
Year:
2023
Authors:
Jia, Jie-Min & Jin, Yuxiao
Affiliation:
School of Life Sciences · China

Abstract

It is challenging to establish animal models to mimic perinatal arterial ischemic stroke. Here, we provided two approaches that precisely occlude rodent pups' distal middle cerebral artery of rodent pups at any postnatal age. One uses magnetic nanoparticles to generate platelet-rich thrombus, and the other utilizes magnetized red blood cells (mRBCs) to generate an erythrocyte-rich embolus. Both approaches result in focal cerebral ischemia followed by controllable reperfusion while requiring no arterial surgery.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/36715928/