Molecular and Seroprevalence of Toxoplasmosis in Goats' Blood and Milk in Iraq.

Abstract

Toxoplasmosis is one of the most acute parasitic and zoonotic infections, which causes severe economic losses in animals due to abortion and reproductive problems worldwide. Therefore, this study was conducted in Baghdad province to detect the prevalence of Toxoplasmosis in blood and milk samples of 384 adult female goats using the serological indirect-enzyme-linked immunosorbent assay (iELISA) and the molecular polymerase chain reaction (PCR) test. The positive iELISA results were 20.57% in sera and 5.99% in milk samples. Regarding cross-classification results, the iELISA results revealed that 5.73% of goats were positive by testing both sera and milk samples, 14.32% and 0.26% were positive for testing sera and milk only, respectively, and 79.69% were negative by testing sera and milk. Targeting the B1 gene, the total positive results of the PCR assay showed that 13.92% and 30.43% of blood and milk samples, respectively, were positive at 546bp. Concerning cross-classification results, the total positive goats by testing of both sera and milk was 8.86%, while 5.06% of goats were positive only for testing of blood, and 86.08% were negative for testing of both samples. At the same time, no positive PCR results were detected in milk samples. In conclusion, there is a wide prevalence and incidence of Toxoplasmosis among goats in study areas. Furthermore, studies are essential to detect the parasite in different ages and sexes of goats and other domestic and wild animals using ELISA as a reliable, automated, and rapid test and PCR as a highly confirmative test.