Molecular characterization and genetic diversity ofandof cattle in Thailand.

Abstract

andare the most common tick-borne parasites that cause bovine babesiosis which effects livestock production, leading to economic losses in tropical and subtropical areas of the world. The aims of this study were to determine the molecular detection, genetic diversity and antigenicity prediction ofbased on spherical body protein 2 () gene andbased on rhoptry-associated protein 1a () gene in cattle in Thailand. By PCR assay, the molecular detection ofandinfection revealed levels of 2.58% (4/155) and 5.80% (9/155), respectively. The phylograms showed thatandsequences displayed 5 and 3 clades with similarity ranging between 85.53 to 100% and 98.28 to 100%, respectively, when compared within Thailand strain. Diversity analysis ofandsequences showed 18 and 4 haplotypes, respectively. The entropy analysis illustrated 104 and 7 polymorphic sites ofandnucleic acid sequences, respectively, while those ofandamino acid sequences showed 46 and 4 high entropy peaks, respectively. Motifs analysis exhibited the distribution and conservation amongandsequences. The continuous and discontinuous B-cell epitopes have also been evaluated in this work. Therefore, our findings may be used to ameliorate the understanding inputs of molecular phylogeny, genetic diversity and antigenicity ofandThailand stains.