Molecular characterization and genetic variability offrom naturally infected buffalo calves for the first time in Bangladesh.

Abstract

is one of the deadliest parasite of buffalo calves in Bangladesh. This study was conducted to explore genetic variability within and among thepopulations in buffalo calves of Bangladesh. Genomic DNA was extracted,andgene were amplified and sequenced. Distinct 29, 21 uniqueand 24genotypes were detected among theof different geographic regions. These three gene genotypes similarities ranged from 97 to 99%, when these were compared to best hit scoringsequences retrieved from GenBank. A total of 12 and 6 unique haplotypes were detected forandgene sequences. The average nucleotide and haplotype diversity forandwere 0.0931 & 0.89493 and 0.00658 & 0.77895 respectively and the recorded values were more dispersed than previously published values. The pairwise Nst values ranged from −0.050 to 0.602 and Fst from −0.050 to 0.600 between all thegenotypes indicated huge genetic differentiation which were reportedly higher than other published reports Fst values. This is the first report ofon the basis ofgene in Bangladesh. The study findings will be helpful for further extensive epidemiological studies regarding anthelmintic resistance, control and prevention ofinfection in buffalo calves.