Peer-reviewed veterinary case report
Molecular characterization and identification of Blastocystis and its subtypes from raccoon (Procyon lotor) in north of Iran.
- Journal:
- Parasitology research
- Year:
- 2020
- Authors:
- Mohammad Rahimi, Hanieh et al.
- Affiliation:
- Research Institute for Gastroenterology and Liver Diseases
Abstract
Blastocystis is a zoonotic protozoan parasite frequently identified in the intestinal tract of humans and a vast variety of animals, worldwide. Here, we assessed the prevalence of Blastocystis and its subtypes in stool samples of raccoons. Stool samples from 30 raccoons were collected. Total DNA was extracted, and the barcoding region of the small subunit ribosomal rRNA (SSU rRNA) gene was amplified and sequenced. Specific fragment for Blastocystis was successfully amplified in five samples (16.66%). Sequencing analysis revealed ST1, ST2, and ST3 among 1, 2, and 2 Blastocystis-positive samples. Our results documented the presence of Blastocystis subtypes 1-3 in raccoons. Subtype 1 showed higher similarity to the human isolates of Blastocystis. However, it seems that raccoons may emerge as reservoirs for Blastocystis and may be linked to zoonotic transmission of the protist.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/32577820/