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Peer-reviewed veterinary case report

Molecular characterization of a canine coronavirus NA/09 strain detected in a dog's organs.

Journal:
Archives of virology
Year:
2012
Authors:
Ntafis, Vasileios et al.
Affiliation:
Department of Anatomy and Physiology of Farm Animals
Species:
dog

Abstract

In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of sequence identity with the pantropic strain CB/05, indicating the presence of CB/05-like pantropic strains in Greece. The absence of the 38-nucleotide deletion in ORF3b, which is characteristic of CB/05, indicates the need to identify new genetic markers for pantropic variants of CCoV, probably in the spike-protein gene region.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/22002680/