Peer-reviewed veterinary case report
Molecular cloning of a Mycoplasma meleagridis-specific antigenic domain endowed with a serodiagnostic potential.
- Journal:
- Veterinary microbiology
- Year:
- 2007
- Authors:
- Mardassi, B Ben Abdelmoumen et al.
- Affiliation:
- Laboratoire des Mycoplasmes
- Species:
- bird
Abstract
A recombinant phage library harbouring Mycoplasma meleagridis (MM) genomic DNA fragments was generated in the bacteriophage lambda gt11 expression vector. The library was screened for expression of MM specific antigens with a polyclonal antiserum that had been preadsorbed with antigens of the most common unrelated avian mycoplasma species. A 49-amino acid antigenic domain unique to MM was isolated, expressed in Escherichia coli, and its serodiagnostic potential was demonstrated. An antiserum raised against this MM-specific antigenic domain recognized a cluster of seven membrane-associated MM proteins with molecular masses ranging from 34 to 75 kDa. Overall, this study resulted in the identification of a potent serodiagnostic tool and revealed the complex antigenic nature of MM.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/16973309/