Molecular detection of Anaplasma spp. in dairy cattle in southern Xinjiang, China.

Abstract

Bovine anaplasmosis is caused by a group of obligate intracellular bacteria belonging to the genus Anaplasma, which are transmitted by ticks. This study was conducted to determine the prevalences and molecular characterization of Anaplasma spp. in dairy cattle in the upper reaches of the Tarim River in Xinjiang, China. Using polymerase chain reaction (PCR) and sequencing approaches, DNA of Anaplasma spp. was detected in 16 of 493 (3.2%) blood samples from dairy cattle. Positive rates were 0.2% (1/493), 0.4% (2/493), 0.2% (1/493), 2.4% (12/493) and 2.4% (12/493) for A. bovis, A. ovis, A. phagocytophilum like strain, A. phagocytophilum and A. platys like strain, respectively. Anaplasma phagocytophilum and A. platys like strain co-infection was detected in 12 samples. To our knowledge, this is the first report of A. ovis infection in dairy cattle in Xinjiang. This study provides new data on the prevalences of Anaplasma spp. in cattle in Xinjiang, which will help to formulate appropriate control strategies for these pathogens in this area.