Peer-reviewed veterinary case report
Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil.
- Journal:
- BMC veterinary research
- Year:
- 2016
- Authors:
- Cezar, Renata Duarte da Silva et al.
- Affiliation:
- Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco - UFRPE) · Brazil
Abstract
BACKGROUND: The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of Pernambuco, Brazil, by real-time quantitative PCR (qPCR) targeting the region of difference 4 (RD4). Risk factors possibly associated with bovine tuberculosis (BTB) were also evaluated. RESULTS: Of the 802 samples analyzed, one milk (0.25%) and eight blood (2%) samples were positive for M. bovis in the qPCR and their identities were confirmed by sequencing. Animals positive for M. bovis were found in six (30%) of the 20 properties visited. None of the risk factors evaluated were statistically associated with BTB. CONCLUSIONS: M. bovis DNA was detected in one milk sample what may pose a risk to public health because raw milk is commonly consumed in Brazil.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26897524/