Peer-reviewed veterinary case report
Molecular detection of Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' Infection in cats by direct PCR using whole blood without DNA extraction.
- Journal:
- The Journal of veterinary medical science
- Year:
- 2008
- Authors:
- Watanabe, Masashi et al.
- Affiliation:
- School of Veterinary Medicine · Japan
- Species:
- cat
Abstract
Detection of hemotropic Mycoplasma spp. infection was attempted in cats by PCR using whole blood without DNA extraction. A total 46 of 54 (85%) cats with suspected Mycoplasma spp. infection showed a positive reaction, corresponding completely with the results of standard PCR testing. The direct PCR assay was sensitive enough to detect more than 0.0061% parasitemia for ;C. M. haemominutum' and 0.0075% parasitemia for M. haemofelis. These data indicate that the direct PCR assay might be sufficient for use as a tool in clinical examinations.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/18981667/