Peer-reviewed veterinary case report
Molecular detection of Salmonella species in bovine fecal samples.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2013
- Authors:
- Bordonaro, Rebecca et al.
- Affiliation:
- Department of Population and Diagnostic Sciences · United States
Abstract
A study was performed to assess the validity of the BAX automated polymerase chain reaction system (DuPont Nutrition & Health, Wilmington, Delaware) to detect the shedding of Salmonella species in bovine fecal samples. A total of 133 bovine fecal samples that were submitted to the Cornell University Animal Health Diagnostic Laboratory for Salmonella culture were also tested in the BAX system with a modified version of the manufacturer's enrichment protocol. Using culture as the gold standard test, the BAX system was found to have a sensitivity of 85.7% and a specificity of 90.5%. There was excellent agreement (κ = 0.71, standard error = 0.072) and no significant differences between the 2 methods (McNemar χ(2) = 0.180).
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24153032/