Molecular detection ofspp. among wild rats in Surabaya, East Java, Indonesia.

Abstract

BACKGROUND: Humans and animals who have an acute case of diarrhea can be infected withspp. Within the category of water-borne disease, it is a zoonotic disease. The zoonotic diseaseis among the several pathogens carried by wild rats (spp.). The risk of spreading this disease is rather significant in urban environments because rats are often close to people. AIM: This study aims to detect Cryptosporidium spp. infection in wild rats in Surabaya, East Java, Indonesia. METHODS: Through necropsy, a total of 100 wild rats' intestines were sampled for feces. Microscopic observation of the presence ofwas carried out using the float test with a combination of Ziehl Neelsen (ZN) staining. Molecular detection ofspp. positive results used theoocyst wall protein (COWP) gene with polimerase chain reaction method. RESULTS: The results showed that 69 samples were positive for containingspp. oocysts and with ZN staining to confirm the diagnosis, the staining results showedspp. oocysts dark pink with a clear cavity inside with a percentage of 95.65% inand 61.03% in. In residential and densely populated environments the percentage ofspp. amounted to 66.66% and in the market environment amounted to 74.19%. The percentage ofspp. in the North Surabaya region was 42.85%, South Surabaya 100%, West Surabaya 37.5%, East Surabaya 81.39%, and Central Surabaya 65.38%. Molecular detection ofspp. positive results were obtained using the COWP gene 550 bp. CONCLUSION: This study aims to detect Cryptosporidium spp. infection in wild rats in Surabaya, East Java, Indonesia. The high number of cases of cryptosporidiosis in wild rats has the potential to be a reservoir for the spread of the disease. The Cryptosporidium spp can detected with COWP in 550 bp in wild rats in Surabaya, East Java, Indonesia.