Peer-reviewed veterinary case report
Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces.
- Journal:
- Acta parasitologica
- Year:
- 2015
- Authors:
- Carnevale, Silvana et al.
Abstract
Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26203987/