Peer-reviewed veterinary case report
Canine parvovirus types found in dog poop samples from Bogotá
By Galvis, Cristian Camilo et al.·Published in Journal of veterinary science·2022·College of Veterinary Medicine·View original on PubMed →
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Original publication title: Molecular diversity of the VP2 of1 (CPV-2) of fecal samples from Bogotá.
- Species:
- dog
Plain-English summary
A group of puppies and young dogs in Bogota were diagnosed with canine parvovirus (CPV), which can cause severe vomiting and diarrhea. Researchers collected fecal samples from these dogs and found that 47 out of 54 tested positive for the virus. Most of the positive samples were identified as subtype 2a, with a few as subtype 2b, while subtype 2c was not found. This study suggests that the CPV strains in Colombia may be changing in a way that affects how the virus is recognized by the immune system.
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Abstract
BACKGROUND: , also known as canine parvovirus type 2 (CPV-2), is the main pathogen in hemorrhagic gastroenteritis in dogs, with a high mortality rate. Three subtypes (a, b, c) have been described based on VP2 residue 426, where 2a, 2b, and 2c have asparagine, aspartic acid, and glutamic acid, respectively. OBJECTIVES: This study examined the presence of CPV-2 variants in the fecal samples of dogs diagnosed with canine parvovirus in Bogotá. METHODS: Fecal samples were collected from 54 puppies and young dogs (< 1 year) that tested positive for the CPV through rapid antigen test detection between 2014-2018. Molecular screening was developed for VP1 because primers 555 for VP2 do not amplify, it was necessary to design a primer set for VP2 amplification of 982 nt. All samples that were amplified were sequenced by Sanger. Phylogenetics and structural analysis was carried out, focusing on residue 426. RESULTS: As a result 47 out of 54 samples tested positive for VP1 screening, and 34/47 samples tested positive for VP2 980 primers as subtype 2a (n = 30) or 2b (n = 4); subtype 2c was not detected. All VP2 sequences had the amino acid, T, at 440, and most Colombian sequences showed an S514A substitution, which in the structural modeling is located in an antigenic region, together with the 426 residue. CONCLUSIONS: The 2c variant was not detected, and these findings suggest that Colombian strains of CPV-2 might be under an antigenic drift.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/34931505/