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Peer-reviewed veterinary case report

Blood test results and parasite detection in stored dog samples

By Santos, Fernanda Barbosa Dos et al.·Published in Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria·2020·Departamento de Microbiologia e Parasitologia·View original on PubMed

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Original publication title: Molecular evaluation of piroplasms and hematological changes in canine blood stored in a clinical laboratory in Niterói, Rio de Janeiro.

Species:
dog

Plain-English summary

A group of dogs in Brazil tested positive for a blood infection caused by a parasite called Babesia vogeli, which can lead to symptoms like anemia and low platelet counts. Out of 31 blood samples, 24 dogs were found to be infected, with many showing signs of anemia and a significant number having low platelet levels. This study highlights the prevalence of Babesia vogeli in dogs and emphasizes the importance of monitoring for these blood-related issues. Treatment typically involves medications to combat the infection and support the dog's overall health.

People also search for: dog anemia treatment · Babesia vogeli symptoms in dogs · low platelet count in dogs

Abstract

Piroplasm species were analyzed by molecular tools in total 31 blood samples from positive dogs, previously checked by stained slides, stored until DNA extraction between 2016 to 2018 in the laboratory Clinical Analyzes in Niterói, Rio de Janeiro. The piroplasms were identified by PCR, targeting the 18S rRNA gene and sequencing. From the total number of samples only 24 (77.4%) were positive and show adequate nucleotide sequences for interpretation with identity between 93%-100% with Babesia vogeli in compared to the sequences isolated of infected dogs from other states in Brazil deposited on GenBank. Most of dogs infected with B. vogeli had anemia (62.5%) and thrombocytopenia (95.8%). The findings of this study are compatible with previous reports in the literature and highlight B. vogeli as the most incriminated species in canine piroplasmosis in Brazil, and thrombocytopenia the hematological alteration most frequently identified in this infection. It is important to note that this is the first study involving the molecular characterization of piroplasms in the metropolitan region of Rio de Janeiro, based on PCR followed by sequencing.

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Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/32756775/