Molecular Phylogenetics of(Digenea: Heterophyidae) Originated from Freshwater Fish from Chiang Mai Province, Thailand.

Abstract

This study aimed to investigate the morphology and reconstruct the phylogenetic relationships oforiginating from 5 species of freshwater fish, i.e.,, and, in Chiang Mai province, Thailand. Sequence-related amplified polymorphism (SRAP) and phylogeny based on internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (CO1) were performed. The results showed similar morphologies of adultfrom day 5 after infection in chicks.originated from 4 species of freshwater fish had the same number of circumoral spines on the oral sucker, except for those fromwhich revealed 34 circumoral spines. The phylogenetic tree obtained from SRAP profile and the combination of ITS2 and CO1 sequence showed similar results that were correlated with the number of circumoral spines in adult worms. Genetic variability ofalso occurred in different species of freshwater fish hosts. However, more details of adult worm morphologies and more sensitive genetic markers are needed to confirm the species validity ofwith 34 circumoral spines originating fromin the future.