Peer-reviewed veterinary case report
Blood test finds GM1 gangliosidosis carriers in Shiba dogs
By Yamato, Osamu et al.·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2008·Department of Veterinary Clinical Sciences, Japan·View original on PubMed →
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Original publication title: Molecular screening of canine GM1 gangliosidosis using blood smear specimens after prolonged storage: detection of carriers among shiba dogs in northern Japan.
- Species:
- dog
Plain-English summary
A group of Shiba dogs in northern Japan was tested for GM1 gangliosidosis, a genetic condition that can affect their health. Researchers used blood samples that had been stored for a long time and found that 2 out of 68 dogs were carriers of the disease, while the rest were healthy. The study indicates that blood smears can still be useful for testing even after being stored, as long as they contain enough blood and are kept properly. This suggests that GM1 gangliosidosis might be more common in Shiba dogs in that region than previously thought.
People also search for: Shiba dog genetic testing · GM1 gangliosidosis in dogs · carrier testing for Shiba Inu
Abstract
Molecular screening of GM1 gangliosidosis in Shiba dogs was carried out in northern Japan using blood smear specimens after prolonged storage. Of 125 specimens obtained from 3 veterinary teaching hospitals for this screening, 68 specimens (54%) were adequate for direct amplification in a polymerase chain reaction (PCR)-based DNA test, and the percentage of adequacy was different at each hospital (34%, 73%, and 100%), suggesting that the amount of blood on the smear and the storage condition of specimens may affect adequacy. Of the 68 dogs examined, 2 dogs (2.9%) were heterozygous carriers for this disease and the other dogs were all genotypically normal. The results suggest blood smear specimens can be useful for PCR testing after prolonged storage provided specimens contain a generous amount of blood and have been adequately stored. The study also suggests that GM1 gangliosidosis may be widely prevalent in the Shiba dog population in northern Japan.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/18182512/