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Peer-reviewed veterinary case report

Molecular Study of the G1 Haplotypes of Echinococcus granulosus from Iran Based on Cytochrome C Oxidase (Subunit 1) Sequence.

Journal:
Turkiye parazitolojii dergisi
Year:
2015
Authors:
Esmaelizad, Majid et al.
Affiliation:
Department of Central Laboratory

Abstract

OBJECTIVE: In this study, we attempted to identify new Echinococcus granulosus isolates in the North West provinces of Iran based on the mitochondrial cytochrome c oxidase subunit 1 (CO1) sequence. METHODS: Twenty-nine hydatid cysts from sheep and goats were collected. Genomic DNAs were extracted, and a partial sequence of the CO1 gene was amplified. Polymerase chain reaction products were cloned and sequenced with M13 primers in both directions. RESULTS: All Iranian isolates were located in G1 and G3 genotypes. For the first time, a new G1 haplotype in two Iranian isolates were identified. CONCLUSION: It seems that this new haplotype was transmitted from Jordan to Iran or vice versa.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/26809915/