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Peer-reviewed veterinary case report

Monitoring α-Synuclein Proteotoxicity in Drosophila Models.

Journal:
Methods in molecular biology (Clifton, N.J.)
Year:
2019
Authors:
Szabo, Aron & Tofaris, George K
Affiliation:
Institute of Genetics

Abstract

Parkinson's disease is the second most common neurodegenerative disease without cure. It is characterized by α-synuclein accumulation and aggregation in dopaminergic and other types of neurons. Because α-synuclein accumulation leads to a toxic gain of function, its ectopic expression in Drosophila has been a useful in vivo model for testing modifiers of its toxicity. This chapter describes four assays: the rapid iterative negative geotaxis, rough eye phenotype, quantification of dopaminergic neuronal loss, and measurements of circadian effects.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/30771179/