Mouse Electroacupuncture Fixation Device Fabrication for Electroacupuncture Pretreatment in Diabetic Cardiomyopathy Mouse Model.

Abstract

Diabetic cardiomyopathy (DCM) is a complication of diabetes that can progress to heart failure and is limited by the use of medications. Electroacupuncture is an effective way as a traditional Chinese medicine external treatment method to treat diabetic cardiomyopathy. A mouse electroacupuncture fixation device can easily and gently fix mice during electroacupuncture treatment and expose acupoints on the abdomen and limbs of mice. This article describes the methods used to fabricate the mouse electroacupuncture fixation device with all the steps and precautions, establishing a mouse model of diabetic cardiomyopathy with comprehensive measures to comprehensively record changes in blood biochemical indices such as blood glucose. The experiment was done with 3 groups: a control group, a model group, and a pretreatment group, with 10 mice in each group. The pretreatment group was treated by Biaoben acupoints electroacupuncture pretreatment once every alternate day for a total of 7 sessions. After electroacupuncture, the mice in the pretreatment group showed a decrease in blood glucose accompanied by a reduction in the symptoms of diabetes.