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Peer-reviewed veterinary case report

Mouse Model to Study Salmonella-Induced Colitis.

Journal:
Methods in molecular biology (Clifton, N.J.)
Year:
2022
Authors:
Ehrhardt, Katrin & Grassl, Guntram A
Affiliation:
Institute of Medical Microbiology and Hospital Epidemiology and German Center for Infection Research (DZIF) · Germany
Species:
rodent

Abstract

Salmonella efficiently colonizes the cecum and proximal colon of mice where it induces inflammation resulting in colitis. To study intestinal infection of non-typhoidal Salmonella enterica serovars in mice, the colonization resistance of the intestine is overcome by transiently reducing the gut microbiota by an oral antibiotic treatment 1 day prior to infection with Salmonella. The in vivo colitis model is crucial for understanding the role of mucosal host defenses, analysis of histopathological changes, and the identification of host and bacterial factors leading to acute infections or facilitating bacterial persistence.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/35619036/