Peer-reviewed veterinary case report
Dog skin yeast infection with multi-azole-resistant Malassezia strain
By Kano, Rui et al.·Published in Medical mycology·2019·Department of Veterinary Pathobiology, Japan·View original on PubMed →
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Original publication title: Multi-azole-resistant strain of Malassezia pachydermatis isolated from a canine Malassezia dermatitis.
- Species:
- dog
Plain-English summary
A dog with skin problems caused by a yeast infection was found to have a strain of Malassezia pachydermatis that was resistant to common antifungal medications. The dog's condition didn't improve with standard treatment using itraconazole, a common antifungal. However, when a different medication called FK506 was added to the treatment, it helped make the itraconazole more effective. This combination therapy showed promise in treating the resistant strain, which had specific genetic mutations that contributed to its resistance.
People also search for: dog skin infection treatment · Malassezia dermatitis in dogs · antifungal treatment for dog yeast infection
Abstract
In the case presented here, we describe the isolation of an azole-resistant strain of M. pachydermatis from a canine Malassezia dermatitis. The isolate (NUBS18001) from this case exhibited a minimum inhibitory concentration (MIC) of 320 μg/ml to itraconazole (ITZ) by broth microdilution (BM) assay, >32 μg/ml to ITZ by E-test, and >32 μg/ml to KTZ by E-test. Synergistic effects between FK506 and ITZ in the azole-resistant strain was evaluated using the microdilution checker-board method. The ITZ-resistant strain exhibited MICs of 320 μg/mL of ITZ alone and 5 μg/ml of FK506 alone; the addition of FK506 attenuated the ITZ MIC to 2.5 μg/ml, yielding an ITZ FICI value of 0.507. This result suggested that the combination of ITZ and FK506 exerted an additive effect against the ITZ-resistant strain. To understand the other mechanism inferred to be present in our multi-azole-resistant strain, we sequenced the ERG11 gene from this isolate, and detected missense mutations (A412G and C905T) in the sequence of the ERG11 open reading frame (ORF). To the best of our knowledge, this work is the first report that a multi-azole-resistant M. pacydermatis strain contains mutations in ERG11.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/29800467/