Multi-drug resistance Trichophyton indotineae in a stray dog.

Abstract

A 2.5-year-old stray dog showed signs of hair loss, mild skin crusting, and redness on extremities and trunk. The etiologic agent was confirmed as Trichophyton indotineae by sequencing of ITS region. Using the Clinical and Laboratory Standards Institute (CLSI M38-A3) guideline, antifungal susceptibility testing showed multidrug resistance phenotype against terbinafine (16 μg/mL), itraconazole, and some other tested antifungals (minimum inhibitory concentration, MIC≥16 μg/mL). However, luliconazole was found to be active in- vitro (0.016 μg/mL). Upon further studies, sequencing of SQLE gene showed an amino acids substitution of Phe397Leu and Ala448Thr, which is potentially linked to terbinafine resistance in Trichophyton species.