Peer-reviewed veterinary case report
Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2021
- Authors:
- Preuss, Jonathan M et al.
- Affiliation:
- Institute of Comparative Molecular Endocrinology (CME) · Germany
Abstract
The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA). Bead-based detection systems allow the simultaneous detection of multiple analytes using minimal sample volumes. Here we describe the use of a multiplex cytokine, chemokine, and growth factor assay for mouse cytokines in a 96-well format. This assay is based on antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition. Final assay readout provides the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/33420994/