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Peer-reviewed veterinary case report

Multiplex PCR to Differentiate Monkeypox Virus Clades.

Journal:
Emerging infectious diseases
Year:
2026
Authors:
Williams, Christopher T et al.

Abstract

We designed a multiplex quantitative PCR to differentiate monkeypox virus clades. For clinical samples collected in the United Kingdom and Nigeria, sensitivity was 78% (95% CI 67.67%-86.14%) and specificity 94% (95% CI 80.84%-99.30%); for samples with cycle thresholds <35, sensitivity was 98% (95% CI 91.72%-99.96%) and specificity 94% (95% CI 80.84%-99.30%).

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Original publication: https://pubmed.ncbi.nlm.nih.gov/41663215/