Peer-reviewed veterinary case report
Detecting feline sporotrichosis from preserved skin samples using
By Luiz, Raul Leal Faria et al.·Published in Frontiers in veterinary science·2021·Oswaldo Cruz Institute, Brazil·View original on PubMed →
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Original publication title: Nested PCR for the Diagnosis of Feline Sporotrichosis From Formalin-Fixed and Paraffin-Embedded Samples Using Different DNA Extraction Protocols.
- Species:
- cat
Plain-English summary
A group of cats with skin lesions was tested for sporotrichosis, a fungal infection that can cause chronic skin problems. Researchers used a special DNA test called nested PCR to identify the fungus in tissue samples taken from the cats. They found that using a specific method to extract DNA from the samples worked best, achieving a 50% success rate in detecting the infection in the cats' skin. This technique could help veterinarians diagnose sporotrichosis more effectively in cats with similar symptoms.
People also search for: cat skin lesions treatment · sporotrichosis in cats · how to diagnose cat fungal infections
Abstract
Sporotrichosis is a chronic, cosmopolitan granulomatous mycosis that affects humans and animals. The infection is caused by the dimorphic fungisp. The aims of the present study were to evaluate, standardize and validate a nested PCR technique using two DNA purification kits for the extraction of DNA from formalin fixed and paraffin-embedded tissues (FFPE) forsp. detection. FFPE mycological culture pellet samples of differentspecies () were used as positive controls and clinical FFPE tissue samples of animals positive forsp.,andsp. were used as negative controls. Ten clinical FFPE skin samples from cats with sporotrichosis were used to validate the nested PCR. These samples were cut into two distinct paraffin sectioning protocols (5 and 16 μm thick). The paraffin sections were subjected to two different DNA extraction kits (chemical and thermal extractions). A nested PCR was performed on the extracted DNA to identify the genus. The chemical extraction protocol with the 5 μm thick paraffin section was more effective in extracting DNA fromsp. from FFPE samples and the nested PCR technique showed the highest sensitivities (100% in the positive controls and of 50% in the skin samples of cats) and specificity (100%). Therefore, the nested PCR using this protocol has great potential to be applied insp. diagnosis in FFPE samples of cats.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/35071377/