Neuroprotective effect of, a flavonoid, sudachitin in mice stroke model.

Abstract

A flavonoid, sudachitin, has been reported to show some beneficial health effects, including as an anti-inflammatory in LPS-stimulated macrophages, as well as improving glucose and lipid metabolism in mice fed a high-fat diet. In this study, we investigated the neuroprotective effect of sudachitin in the transient middle cerebral artery occlusion (tMCAO) mouse model. After daily pre-treatment of vehicle or sudachitin (5 or 50&#xa0;mg/kg) for 14&#xa0;days, mice (n&#xa0;=&#xa0;76) were subjected to a sham operation or tMCAO for 45&#xa0;min, and on the following days, they were treated daily with vehicle or sudachitin. The administration of sudachitin significantly reduced (p&#xa0;<&#xa0;0.05) cerebral infarct volume and attenuated apoptosis, 5&#xa0;days after tMCAO. Neurological impairment improved, the expression of an oxidative stress marker, 4-HNE, decreased, and the Sirt1/PGC-1&#x3b1; pathway was activated 5&#xa0;days after tMCAO in the sudachitin-treated group. This is the first report to demonstrate the neuroprotective effect of sudachitin in cerebral ischemia/reperfusion injury mice model, probably by activating the Sirt1/PGC-1&#x3b1; axis. Sudachitin may be a promising supplement or therapeutic agent for reducing injury caused by ischemic strokes.