Neutrophil Myeloperoxidase Index in Dogs With Babesiosis Caused by.

Abstract

Babesiosis caused by the virulent tick-borne hemoprotozoan,, results in a marked systemic inflammatory host response in dogs. Neutrophils form part of the innate immune response and contains myeloperoxidase (MPO) as the predominant component of the neutrophil lysosomal protein in azurophilic granules. The neutrophil myeloperoxidase index (MPXI), determined on the ADVIA hematology analyzer, is a quantitative estimate of intracellular MPO content. Objectives of this study were to: (a) compare MPXI in dogs with babesiosis with healthy control dogs; (b) compare MPXI in dogs that died from babesiosis with dogs that survived and controls; and (c) correlate the MPXI with the previously determined segmented and band neutrophil count and cytokine concentrations in dogs with babesiosis. Data for 140 dogs naturally infected withand 20 healthy control dogs were retrospectively evaluated. Neutrophil counts and MPXI were determined on an ADVIA 2120 analyzer. Cytokine concentrations [interleukin (IL)-2, IL-6, IL-8, IL-10, IL-18, granulocyte-macrophage colony stimulating factor (GM-CSF), and monocyte chemo-attractant protein-1 (MCP-1)] were determined using a canine-specific multiplex immunoassay. The mortality rate of thedogs was 11% (15/140). MPXI was significantly higher indogs (= 0.033), and innon-survivors (= 0.011), compared with healthy control dogs. Indogs a significant positive correlation was found between MPXI and IL-10 (= 0.211,= 0.039) and a significant negative correlation was found between MPXI and IL-8 (= -0.350,< 0.001). Innon-survivors, significant positive correlations were found between MPXI and IL-2 (= 0.616= 0.033), IL-6 (= 0.615,= 0.033), IL-18 (= 0.613,= 0.034), GM-CSF (= 0.630,= 0.028), and MCP-1 (= 0.713,= 0.009). Insurvivors, a significant negative correlation was found between MPXI and IL-8 (= -0.363,= 0.001). MPXI was correlated with pro-inflammatory cytokines indogs that died. The potential of MPXI as a novel marker of inflammation and prognosis in dogs infected with, thus warrants further investigation.