Peer-reviewed veterinary case report
New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses.
- Journal:
- Journal of virological methods
- Year:
- 2017
- Authors:
- Naguib, Mahmoud M et al.
- Affiliation:
- The Federal Research Institute for Animal Health · Germany
- Species:
- bird
Abstract
In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/28336367/