Peer-reviewed veterinary case report
One-step reverse transcription-loop-mediated isothermal amplification assay for sensitive and rapid detection of porcine kobuvirus.
- Journal:
- Journal of virological methods
- Year:
- 2014
- Authors:
- Li, Xinqiong et al.
- Affiliation:
- College of Veterinary Medicine · China
Plain-English summary
Researchers have developed a new test to quickly and accurately detect porcine kobuvirus (PKoV), a virus linked to stomach issues in pigs. This test, called RT-LAMP, is more sensitive than previous methods, allowing it to find the virus even when it's present in very small amounts. In a study of sick piglets in Sichuan province, the test successfully identified the virus in about 87% of the samples tested. The results suggest that this new method is easy to use and could be very helpful for diagnosing PKoV in both farms and labs. Overall, the RT-LAMP test works well for detecting this virus in pigs.
Abstract
Porcine kobuvirus (PKoV) is associated with swine gastroenteritis, but its pathogenesis is uncertain. In this study, a rapid one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) method for the detection of PKoV is developed. A set of four primers specific to six regions within the PKoV 3D gene was designed for the RT-LAMP assay using total RNA extracted from PKoV-infected tissues. The reaction temperature and time for this assay were optimized. Compared with reverse-transcription PCR, RT-LAMP was able to detect PKoV at a 100-fold lower dilution. No cross-reaction was observed with other similar viruses, indicating that the assay is highly specific for PKoV. To investigate the prevalence of PKoV in symptomatic pigs in Sichuan province, the newly developed method was used to detect PKoV in a panel of clinical specimens, yielding a positive rate of 86.7% (144/166) in piglets. The results showed that the RT-LAMP assay is highly feasible in clinical settings. The data confirm that the RT-LAMP assay is rapid, simple and cost-effective and is particularly suitable for simple diagnosis of PKoV both in the field and in the laboratory.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24972366/