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Peer-reviewed veterinary case report

Optic Nerve Crush Injury in Rodents to Study Retinal Ganglion Cell Neuroprotection and Regeneration.

Journal:
Methods in molecular biology (Clifton, N.J.)
Year:
2023
Authors:
Ahmed, Zubair
Affiliation:
Institute of Inflammation and Ageing · United Kingdom

Abstract

Optic nerve crush injury is a useful model for studying the response of central nervous system neurons (CNS) to injury. A particular focus of this model has been to elucidate therapeutic factors in promoting neuroprotection and axon regeneration after injury. Here we describe a step-by-step protocol in accessing the optic nerve and creating a crush injury. This can be used to create a reproducible model to study the response of retinal ganglion cells (RGC), the main projection neurons of the eye, to injury.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/37558963/