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Peer-reviewed veterinary case report

Optimised extraction of Erythronium sibiricum bulb polysaccharides and evaluation of their bioactivities.

Journal:
International journal of biological macromolecules
Year:
2016
Authors:
Chen, Chunli et al.
Affiliation:
Pharmacy College of Xinjiang Medical University · China

Abstract

In this study, the extraction of Erythronium sibiricum bulb polysaccharides (ESBP) through hot water decoction was optimised using response surface methodology (RSM) and a three-level, four-factor Box-Behnken design. The optimum extraction conditions were as follows: extraction time of 4.28h, extraction temperature of 90°C, ratio of liquid to raw material of 37 mL/g and extraction cycle number of three. The experimental yield (37.25%±0.17%) agreed with the predicted value of the RSM model (37.465%). Preliminary ESBP characterisation was conducted through physicochemical analysis. Biological activity test results showed that ESBP exhibited antioxidant activities and excellent anti-inflammatory and analgesic activities, indicating its potential as an anti-inflammatory and analgesic agent.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/26526177/