Peer-reviewed veterinary case report
Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue.
- Journal:
- Applied and environmental microbiology
- Year:
- 2009
- Authors:
- Carroll, James et al.
- Affiliation:
- Department of Biological Science
Abstract
A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 10(4) to 10(8) CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this method has applications for high-throughput screening against M. avium subsp. paratuberculosis.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/19837835/