Peer-reviewed veterinary case report
Oxygen Assessment in Tumors In Vivo Using Phosphorescence Lifetime Imaging Microscopy.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2024
- Authors:
- Komarova, Anastasia D et al.
- Affiliation:
- Institute of Experimental Oncology and Biomedical Technologies
Abstract
The oxygen level in a tumor is a crucial factor for its development and response to therapies. Phosphorescence lifetime imaging (PLIM) with the use of phosphorescent oxygen probes is a highly sensitive, noninvasive optical technique for the assessment of molecular oxygen in living cells and tissues. Here, we present a protocol for microscopic mapping of oxygen distribution in a mouse tumor model in vivo. We demonstrate that PLIM microscopy, in combination with an Ir(III)-based probe, enables visualization of cellular-level heterogeneity of tumor oxygenation.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/38319571/