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Peer-reviewed veterinary case report

<p>Cigarette smoke combined withinduce damage to the air&#x2011;blood barrier in chronic obstructive pulmonary disease rats via the MAPK/NF&#x2011;&#x3ba;B/I&#x3ba;B&#x3b1; pathway</p>.

Journal:
Molecular medicine reports
Year:
2026
Authors:
Tian, Yange et al.
Affiliation:
Henan University of Chinese Medicine · China
Species:
rodent

Abstract

<p>Chronic obstructive pulmonary disease (COPD) is a respiratory disorder characterized by progressive dyspnea. Damage to the lung air&#x2011;blood barrier is a major cause of progressive dyspnea observed in COPD. Although cigarette smoke inhalation and repetitive bacterial infection cause and exacerbate COPD, their specific effects on the air&#x2011;blood barrier remain to be fully elucidated. The present study explored the effects of the air&#x2011;blood barrier in a COPD rat model induced by cigarette smoke inhalation and repetitive bacterial infection. From weeks 1&#x2011;8, Sprague&#x2011;Dawley rats were treated with cigarette smoke inhalation and repeatedexposure. At the end of week 8, lung function, pulmonary pathology, mucin content, inflammation, oxidative stress and MAPK/NF&#x2011;&#x3ba;B/I&#x3ba;B&#x3b1; pathway indicators were detected in rats. Lung function parameters, including tidal volume, peak expiratory flow and 50% tidal volume expiratory flow showed significant decreases in COPD model rats. The pulmonary organizational structure and ultrastructure of the air&#x2011;blood barrier were also markedly damaged in COPD model rats. Due to cigarette smoke andexposure, the expression of IL&#x2011;6, malondialdehyde, mucoprotein (MUC)5AC, MUC5B, matrix metallopeptidase&#x2011;9 and angiopoietin&#x2011;2 increased in COPD rats, while the expression of IL&#x2011;10, tissue inhibitor of metalloproteinases&#x2011;1, heme oxygenase&#x2011;1, zonula occludens&#x2011;1, claudin&#x2011;5, aquaporin&#x2011;5, surfactant protein&#x2011;D and superoxide dismutase significantly decreased. Subsequently, cigarette smoke exposure andinfection increased the levels of phosphorylated&#x2011;(p&#x2011;)p38, p&#x2011;ERK, p&#x2011;JNK, p&#x2011;p65 and p&#x2011;I&#x3ba;B&#x3b1;. The present study provided notable evidence that cigarette smoke andexposure exacerbated the destruction of the air&#x2011;blood barrier in COPD via the MAPK/NF&#x2011;&#x3ba;B/I&#x3ba;B&#x3b1; pathway.</p>.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/41543188/